武汉大学化学与分子科学学院博士生导师简介：袁必锋

　　考博考生生准备要参加博士研究生考试时，必须要先确定准备攻读博士的相关专业，然后选择该专业有招生需求的学校，接下来应该联系博士生导师，只有当博士生导师同意考生报考，考博生才可以报考。所以提前了解博士生导师的学术文章及联系方式很重要，新东方在线特整理了各招收博士院校博导的简介及联系方式供考博生参考。

　　袁必锋

　　博士 , 教授

　　研究方向： 色谱-质谱分析 / 生物分析化学

　　联系电话： 化西109B、68755087/13349891309

　　Email: bfyuan@whu.edu.cn

　　教育与研究经历

　　1997. 9 - 2001. 6 武汉大学 生命科学学院 生物化学专业 学士

　　2001. 9 - 2006. 6 武汉大学 生命科学学院 生物物理专业 博士

　　2006. 11 - 2007. 6 新加坡国立大学 生物系 博士后

　　2007. 7 - 2011. 1 美国加州大学河滨分校 化学系 博士后

　　2011. 2 至今 武汉大学 化学与分子科学学院 教授/博导

　　研究领域与兴趣

　　1. 核酸表观遗传修饰(Epigenetic Modifications)

　　核酸表观遗传修饰不仅在基因调控等方面有重要作用，而且在癌症等许多疾病的发生中也扮演着重要角色。本课题组通过建立质谱分析等研究方法，对核酸表观遗传修饰的生理功能进行研究。

　　2. 代谢组分析(Metabolomics)

　　发展基于质谱技术的代谢组学研究方法，结合相应的生物学手段，探索代谢物在疾病发生、发展过程中的生理功能。

　　3. 核酸损伤和修复(Nucleic Acid Damage and Repair)

　　核酸损伤与基因突变、衰老、癌症发生等都有密切的关系。发展质谱分析技术，探索核酸损伤机制，为疾病的防治以及新药的开发提供实验和理论基础。

　　本课题组致力于化学和生物学交叉领域的研究工作，欢迎具有化学或者生物学背景的学生、博士后加入。

　　项目

　　1. 自然科学基金面上项目(21672166)

　　2. 自然科学基金优秀青年基金(21522507)

　　3. 自然科学基金青年基金(21205091)

　　4. 自然科学基金海外及港澳学者联合基金(21228501)

　　5. 国家重点基础研究发展计划(973 计划)子课题(2012CB720601)

　　课程

　　本科生课程：《化学生物学》

　　研究生课程：《生物分析化学》

　　代表性论文

　　Huang, W., Lan, M.D., Qi, C.B., Zheng, S.J., Wei, S.Z., Yuan, B.F.* and Feng, Y.Q. (2016). Formation and determination of the oxidation products of 5-methylcytosine in RNA. Chem Sci. 7, 5495-5502.

　　Zhang, H.Y., Xiong, J., Qi, B.L., Feng, Y.Q.* and Yuan, B.F.* (2016) The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals. Chem Commun (Camb), 52, 737-740

　　Huang, W., Qi, C.B., Lv, S.W., Xie, M., Feng, Y.Q.*, Huang, W.H.* and Yuan, B.F.* (2016) Determination of DNA and RNA Methylation in Circulating Tumor Cells by Mass Spectrometry. Anal Chem. 88, 1378-1384.

　　Tang, Y., Zheng, S.J., Qi, C.B., Feng, Y.Q.* and Yuan, B.F.* (2015) Sensitive and simultaneous determination of 5-methylcytosine and its oxidation products in genomic DNA by chemical derivatization coupled with liquid chromatography-tandem mass spectrometry analysis. Anal Chem, 87, 3445-3452.

　　Chu, J.M., Qi, C.B., Huang, Y.Q., Jiang, H.P., Hao, Y.H., Yuan, B.F.* and Feng, Y.Q.* (2015) Metal Oxide-Based Selective Enrichment Combined with Stable Isotope Labeling-Mass Spectrometry Analysis for Profiling of Ribose Conjugates. Anal Chem, 87, 7364-7372.

　　Shen, F., Huang, W., Huang, J.T., Xiong, J., Yang, Y., Wu, K., Jia, G.F., Chen, J., Feng, Y.Q.*, Yuan, B.F.*, Liu, S.M.* (2015) Decreased N(6)-Methyladenosine in Peripheral Blood RNA From Diabetic Patients Is Associated With FTO Expression Rather Than ALKBH5. J Clin Endocrinol Metab, 100, E148-154.

　　Yuan, B.F.* and Feng, Y.Q. (2014) Recent advances in the analysis of 5-methylcytosine and its oxidation products. TrAC-Trend Anal Chem, 54, 24-35.

　　Xing, X.W., Tang, F., Wu, J., Chu, J.M., Feng, Y.Q., Zhou, X.* and Yuan, B.F.* (2014) Sensitive detection of DNA methyltransferase activity based on exonuclease-mediated target recycling. Anal Chem, 86, 11269-11274.

　　Tang, Y., Xiong, J., Jiang, H.P., Zheng, S.J., Feng, Y.Q.* and Yuan, B.F.* (2014) Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis. Anal Chem, 86, 7764-7772.

　　Qi, B.L., Liu, P., Wang, Q.Y., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Derivatization for liquid chromatography-mass spectrometry. TrAC-Trend Anal Chem, 59, 121-132.

　　Liu, P., Huang, Y.Q., Cai, W.J., Yuan, B.F.* and Feng, Y.Q.* (2014) Profiling of thiol-containing compounds by stable isotope labeling double precursor ion scan mass spectrometry. Anal Chem, 86, 9765-9773.

　　Wang, S.T., Huang, W., Lu, W., Yuan, B.F.* and Feng, Y.Q.* (2013) TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleosides before Detection of 2''-Deoxynucleosides/Low-Abundance 2''-O-Modified Ribonucleosides. Anal Chem, 85, 10512-10518.

　　Tang, Y., Chu, J.M., Huang, W., Xiong, J., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Hydrophilic material for the selective enrichment of 5-hydroxymethylcytosine and its liquid chromatography-tandem mass spectrometry detection. Anal Chem, 85, 6129-6135.

　　Chen, M.L., Shen, F., Huang, W., Qi, J.H., Wang, Y., Feng, Y.Q.*, Liu, S.M.* and Yuan, B.F.* (2013) Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Carcinoma Tissues by Capillary Hydrophilic-Interaction Liquid Chromatography/Quadrupole TOF Mass Spectrometry. Clin Chem, 59, 824-832.

　　Chen, M.L., Liu, Y.L., Xing, X.W., Zhou, X., Feng, Y.Q.* and Yuan, B.F.* (2013) Preparation of a Hyper-cross-linked Polymer Monolithic Column and Its Application to the Sensitive Determination of Genomic DNA Methylation. Chem-Eur J, 19, 1035-1041.

　　Tang, Y., Gao, X.D., Wang, Y., Yuan, B.F.* and Feng, Y.Q.* (2012) Widespread existence of Cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal Chem, 84, 7249-7255.

　　Yuan, B.F., Zhang, J., Wang, H., Xiong, L., Cai, Q., Wang, T., Jacobsen, S., Pradhan, S. and Wang, Y.* (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1. Cancer Res, 71, 1904-1911.

　　Yuan, B.F., Wang, J., Cao, H., Sun, R. and Wang, Y.* (2011) High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing. Nucleic Acids Res, 39, 5945-5954.

　　Yuan, B.F., O''Connor, T.R. and Wang, Y.* (2010) 6-Thioguanine and S(6)-Methylthioguanine Are Mutagenic in Human Cells. ACS Chem. Biol., 5, 1021-1027.

　　Yuan, B.F., Cao, H., Jiang, Y., Hong, H. and Wang, Y.* (2008) Efficient and accurate bypass of N2-(1-carboxyethyl)-2''-deoxyguanosine by DinB DNA polymerase in vitro and in vivo. Proc. Natl. Acad. Sci. U S A., 105, 8679-8684.

　　Yuan, B.F., Zhuang, X.Y., Hao, Y.H. and Tan, Z.* (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb), 6600-6602.

　　发表的全部论文可以从下面的Google Scholar连接查看

　　http://scholar.google.com/citations?hl=enundefinedamp;user=jrzBHhAAAAAJundefinedamp;view_op=list_worksundefinedamp;pagesize=100

　　获奖和荣誉

　　2015 国家自然科学基金优秀青年基金获得者

　　2011 “楚天学者”特聘教授

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