武汉大学药学院博士生导师简介：孙宇辉

　　教学情况

　　● 本科生《分子遗传学》

　　● 硕士研究生《生物药学研究进展》

　　● 博士研究生《生物技术前沿讲座》

　　研究组成员

　　现有讲师2名，博士研究生4名，硕士研究生9名;已毕业博士研究生5名，硕士研究生13名。

　　已发表的学术论文(*：通讯作者，#：第一作者)

　　● Marie E. Yurkovich, Robert Jenkins, Yuhui Sun, Manuela Tosin* and Peter F. Leadlay* (2017) The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase. Chemical Communications 53:2182-2185.

　　● Judith Havemann, Marie E. Yurkovich, Robert Jenkins, Sophia Harringer, Weixin Tao, Shishi Wen, Yuhui Sun, Peter F. Leadlay and Manuela Tosin* (2017) Chemical probing of thiotetronate bio-assembly. Chemical Communications 53:1912-1915.

　　● Hui Hong, Yuhui Sun, Yongjun Zhou, Emily Stephens, Markiyan Samborskyy and Peter F. Leadlay* (2016) Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase. Beilstein Journal of Organic Chemistry 12:2164-2172.

　　● Jianzhao Qi, Dan Wan, Hongmin Ma, Yuanzhen Liu, Rong Gong, Xudong Qu, Yuhui Sun, Zixin Deng and Wenqing Chen* (2016) Deciphering carbamoylpolyoxamic acid biosynthesis reveals unusual acetylation cycle associated with tandem reduction and sequential hydroxylation. Cell Chemical Biology 23:935-944.

　　● Yujie Geng, Zixin Deng and Yuhui Sun* (2016) An insight into the protospacer adjacent motif of Streptococcus pyogenes Cas9 with artificially stimulated RNA-guided-Cas9 DNA cleavage flexibility. RSC Advances 6:33514-33522.

　　● Weixin Tao#, Marie E. Yurkovich#, Shishi Wen#, Karen E. Lebe, Markiyan Samborskyy, Yuanzhen Liu, Anna Yang, Yunkun Liu, Yingchen Ju, Zixin Deng, Manuela Tosin, Yuhui Sun* and Peter F. Leadlay* (2016) A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. Chemical Science 7:376-385.

　　● Yunkun Liu#, Weixin Tao#, Shishi Wen, Zhengyuan Li, Anna Yang, Zixin Deng and Yuhui Sun* (2015) In vitro CRISPR/Cas9 system for efficient targeted DNA editing. mBio 6:e 01714-15.

　　● Hu Zeng, Shishi Wen, Wei Xu, Zhaoren He, Guifa Zhai, Yunkun Liu, Zixin Deng and Yuhui Sun* (2015) Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system. Applied Microbiology and Biotechnology 99:10575-10585.

　　● Chuan Huang#, Fanglu Huang#, Eileen Moison, Junhong Guo, Xinyun Jian, Xiaobo Duan, Zixin Deng, Peter F. Leadlay* and Yuhui Sun* (2015) Delineating the biosynthesis of gentamicin X2, the common precursor of the gentamicin C antibiotic complex. Chemistry & Biology 22:251-261.

　　● Junhong Guo#, Fanglu Huang#, Chuan Huang, Xiaobo Duan, Xinyun Jian, Finian Leeper, Zixin Deng, Peter F. Leadlay* and Yuhui Sun* (2014) Specificity and promiscuity at the branch point in gentamicin biosynthesis. Chemistry & Biology 21:608-618.

　　● Yuhui Sun* (2014) A carbonate architect emerges. Nature Chemical Biology 10:486-487. (Invited Comments)

　　● Yanling Ma, Wei Xu, Jun Zhang, Sihong Zhang, Kui Hong, Zixin Deng and Yuhui Sun* (2014) Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726. Applied Microbiology and Biotechnology 98:8583-8590.

　　● Weixin Tao#, Manghong Zhu#, Zixin Deng and Yuhui Sun* (2013) Biosynthesis of tetronate antibiotics: A growing family of natural products with broad biological activities. Science China Chemistry 56:1364-1371. (Invited Review)

　　● Chompoonik Kanchanabanca#, Weixin Tao#, Hui Hong, Yajing Liu, Frank Hahn, Markiyan Samborskyy, Zixin Deng, Yuhui Sun* and Peter F. Leadlay* (2013) Unusual acetylation-elimination in the formation of tetronate antibiotics. Angewandte Chemie International Edition 52:5785-5788.

　　● Marie E. Yurkovich, Petros A. Tyrakis, Hui Hong, Yuhui Sun, Markiyan Samborskyy, Kohei Kamiya and Peter F. Leadlay* (2012) A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. ChemBioChem 13:66-71.

　　● Jeroen S. Dickschat, Olivia Vergnolle, Hui Hong, Stephen Garner, Susanna R. Bidgood, Hannah C. Dooley, Zixin Deng, Peter F. Leadlay* and Yuhui Sun* (2011) An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis. ChemBioChem 12:2408-2412.

　　注：本论文被 “Nature Chemical Biology, 2011,7(12):856”作为“研究亮点(Research Highlights)”推荐。

　　● Yunlong He, Yuhui Sun, Tiangang Liu, Xiufen Zhou, Linquan Bai* and Zixin Deng (2010) Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification. Applied and Environmental Microbiology 76:3283-3292.

　　● Yuhui Sun*, Frank Hahn, Yuliya Demydchuk, James Chettle, Manuela Tosin, Hiroyuki Osada and Peter F. Leadlay* (2010) In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology 6:99-101.

　　注：本论文被英国Faculty of 1000收录(http://f1000.com/)并获专家点评。

　　● Yuhui Sun, Xinyi He, Jingdan Liang, Xiufen Zhou and Zixin Deng* (2009) Analysis of functions in plasmid pHZ1358 influencing its genetic and structural stability in Streptomyces lividans 1326. Applied Microbiology and Biotechnology 82:303-310.

　　● David H. Kwan#, Yuhui Sun#, Frank Schulz, Hui Hong, Bojana Popovic, Joalice C.C. Sim-Stark, Stephen F. Haydock and Peter F. Leadlay* (2008) Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. Chemistry & Biology 15:1231-1240.

　　● Yuliya Demydchuk#, Yuhui Sun#, Hui Hong, James Staunton, Jonathan B. Spencer and Peter F. Leadlay* (2008) Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. ChemBioChem 9:1136-1145.

　　● Yuhui Sun, Hui Hong, Fraser Gillies, Jonathan B. Spencer and Peter F. Leadlay* (2008) Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics. ChemBioChem 9:150-156.

　　● Barbara M. Harvey, Tatiana Mironenko, Yuhui Sun, Hui Hong, Zixin Deng, Peter F. Leadlay, Kira J. Weissman and Stephen F. Haydock* (2007) Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. Chemistry & Biology 14:703-714.

　　● Yuhui Sun, Hui Hong, Markiyan Samborskyy, Tatiana Mironenko, Peter F. Leadlay and Stephen F. Haydock* (2006) Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. Microbiology 152:3507-3515.

　　● Tiangang Liu, Delin You, Chiara Valenzano, Yuhui Sun, Jialiang Li, Qing Yu, Xiufen Zhou, David E. Cane and Zixin Deng* (2006) Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. Chemistry & Biology 13:945-955.

　　● Yuhui Sun, Xiufen Zhou, Hui Dong, Guoquan Tu, Min Wang, Bofei Wang and Zixin Deng* (2003) A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. Chemistry & Biology 10:431-441.

　　● Yuhui Sun, Xiufen Zhou, Guoquan Tu and Zixin Deng* (2003) Identification of a gene cluster encoding meilingmycin biosynthesis among multiple polyketide synthase contigs isolated from Streptomyces nanchangensis NS3226. Archives of Microbiology 180:101-107.

　　● Yuhui Sun, Xiufen Zhou, Jun Liu, Kai Bao, Guiming Zhang, Guoquan Tu, Tobias Kieser and Zixin Deng* (2002) ‘Streptomyces nanchangensis’, a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters. Microbiology 148:361-371.

　　● Xiuhua Pang, Xiufen Zhou, Yuhui Sun and Zixin Deng* (2002) Physical map of the linear chromosome of Streptomyces hygroscopicus 10-22 deduced by analysis of overlapping large chromosomal deletions. Journal of Bacteriology 184:1958-1965.

　　● Xiuhua Pang, Yuhui Sun, Liu Jun, Xiufen Zhou and Zixin Deng* (2002) A linear plasmid temperature-sensitive for replication in Streptomyces hygroscopicus 10-22. FEMS Microbiology Letters 208:25-28.

　　国际合作

　　剑桥大学生物化学系Herchel Smith讲席教授、英国皇家学会院士(FRS)、英国皇家化学会院士(FRSC)Peter F. Leadlay (http://www.bio.cam.ac.uk/~pflgroup/)

　　博士后招聘

　　本研究组长期招聘具有相关或交叉学科专业研究背景的博士后